Development and Application of the Novel Visual Loop-Mediated Isothermal Amplification of Omp25 Sequence for Rapid Detection of Brucella sp.
نویسندگان
چکیده
منابع مشابه
Rapid detection of infectious bursal disease by loop-mediated isothermal amplification for field analysis
Infectious bursal disease (IBD) is an immunosuppressive, acute and highly contagious illness of growing-poultry stock infected with infectious bursal disease virus (IBDV). It is common in Pakistan, causing potential economic losses throughout the year. The objective of the study is to propose a rapid, sensitive and specific diagnostic tool, and compare it with existing commonly used reverse tra...
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Identification of fusion genes in cancer is essential for pathological diagnosis and clinical therapy. Although methods for detection of fusion genes, such as fluorescence in situ hybridization (FISH) and real-time polymerase chain reaction (PCR), have been developed in last two decades, these methods are not ideal for detection of these genetic alterations owing to their high cost and time-con...
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background and objectives: loop-mediated isothermal amplification is a novel nucleic acid amplification assay providing as a simple diagnostic tool for rapid identification of microbial diseases in developing countries. in this study, a lamp assay was established for yersinia enterocolitica , a leading cause of acute enterocolitis in young children. materials and methods: lamp assay was establi...
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conclusions the analytical sensitivity of lamp and pcr methods, using the designed primers, was 8 times more than any other reported methods. the designed methods are specific and sensitive for detection of h. pylori in different clinical and environmental samples. results the detection limits of the lamp and pcr assay were similar and were 10 fg of pure dna of h. pylori, which is equal to 6 co...
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Background and Aims: considering difficulties in usual laboratory methods in detection of viral infections, improved DNA-based diagnostic techniques are more reliable. Loop mediated isothermal amplification method (LAMP) is a nucleic acid amplification method that amplifies DNA using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. In this study, the...
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ژورنال
عنوان ژورنال: Journal of Animal and Veterinary Advances
سال: 2011
ISSN: 1680-5593
DOI: 10.3923/javaa.2011.2120.2126